Microdissection
Membrane-based (laser) microdissection – developed in the 1990s for the detection of (tumour) specific alterations in solid tumours – has become a life sciences key technology for the analysis of specific cells or subcellular compartments on the genomic, RNA or protein level.
Microdissection: Precise separation of cell structures
Microdissection is a highly precise technique used in modern medical and biological research. This method allows for the separation and analysis of specific cell structures and tissues under a microscope. By utilizing state-of-the-art laser technology, scientists and medical professionals can selectively extract samples without damaging the surrounding cells.
Advantages of microdissection
Microdissection offers numerous advantages over traditional tissue sampling methods:
- Precision: Allows targeted extraction of cells and tissues.
- Preservation of sample quality: Minimizes damage to surrounding structures.
- Efficiency: Enables rapid and accurate analysis of specific cell types.
- Diverse applications: Ranges from cancer research to genetic analysis.
Applications of microdissection
Microdissection is used in various research fields, including:
- Oncology: Identification and analysis of tumor cells.
- Neuroscience: Examination of specific brain regions.
- Genetics: Isolation of DNA, RNA, and proteins from individual cells.
- Pathology: Precise diagnosis of tissue samples.
Methods of laser microdissection
- Use of laser beams: Utilizes laser beams for precise separation of cell structures and tissues.
- Advanced systems: Employs computer-aided systems and artificial intelligence (AI) to enhance accuracy and efficiency (automated microdissection).
Microdissection in practice
Microdissection can be performed on both tissue samples and cell cultures:
- Tissue samples: These are cut into ultra-thin sections using microtomes, carefully fixed, and placed on special slides. Under the microscope, target structures are identified and isolated using appropriate dissection techniques.
- Cell cultures: Individual cells or cell structures are isolated by integrating special devices for cell cultivation directly into the microscope. Desired structures are located and processed using computer assistance.
The obtained samples can then be used for further downstream analyses of molecular structures, such as sequencing or mass spectrometry.
Conclusion
Microdissection is an indispensable technique in modern research. It allows for the detailed examination and analysis of specific cell structures, leading to a better understanding of complex biological processes and diseases.
Learn more about the applications and benefits of microdissection on our website and discover how this technique can advance your research.
Methods of laser microdissection
- Use of laser beams: Utilizes laser beams for precise separation of cell structures and tissues.
- Advanced systems: Employs computer-aided systems and artificial intelligence (AI) to enhance accuracy and efficiency (automated microdissection).
Microdissection in practice
Microdissection can be performed on both tissue samples and cell cultures:
- Tissue samples: These are cut into ultra-thin sections using microtomes, carefully fixed, and placed on special slides. Under the microscope, target structures are identified and isolated using appropriate dissection techniques.
- Cell cultures: Individual cells or cell structures are isolated by integrating special devices for cell cultivation directly into the microscope. Desired structures are located and processed using computer assistance.
The obtained samples can then be used for further downstream analyses of molecular structures, such as sequencing or mass spectrometry.
Conclusion
Microdissection is an indispensable technique in modern research. It allows for the detailed examination and analysis of specific cell structures, leading to a better understanding of complex biological processes and diseases.
Learn more about the applications and benefits of microdissection on our website and discover how this technique can advance your research.
Company
Corporated in 2001, we manufacture and sell premium quality consumables for membrane-based microdissection.